Whole-genome libraries for Drosophila
ثبت نشده
چکیده
wide range of genome manipulations. Ivics and colleagues review the basics of transposon biology and of transposon use in the mouse as a vertebrate model system. The authors discuss the application of transposons to the transgenesis of somatic and germ cells, embryonic stem cells and induced pluripotent stem cells as well as their use in forward genetic screens. Readers are alerted to important considerations when considering transposon systems for genetic manipulations. Review p415
منابع مشابه
RepARK—de novo creation of repeat libraries from whole-genome NGS reads
Generation of repeat libraries is a critical step for analysis of complex genomes. In the era of next-generation sequencing (NGS), such libraries are usually produced using a whole-genome shotgun (WGS) derived reference sequence whose completeness greatly influences the quality of derived repeat libraries. We describe here a de novo repeat assembly method--RepARK (Repetitive motif detection by ...
متن کاملUse of high coverage reference libraries of Drosophila melanogaster for relational data analysis. A step towards mapping and sequencing of the genome.
Three differently made, primary Drosophila cosmid libraries of 16-fold genome coverage have been generated. Also, a jumping library has been created by a new method that takes advantage of methylation differences between genomic DNA and vector. Thirdly, two cDNA libraries have been picked. All these libraries have been arrayed on high-density in situ filters, each containing 9216 clones. As a r...
متن کاملUse of reference libraries and hybridisation fingerprinting for relational genome analysis.
The concept of relational genome analysis by hybridisation has been developed into a working system. Various genomic and cDNA libraries have been generated and are distributed via a reference system. Analysis procedures have been tested successfully in the mapping of the entire Schizosaccharomyces pombe genome. In another test-case for their refinement, analyses on the Drosophila genome are wel...
متن کاملA computational search for box C/D snoRNA genes in the Drosophila melanogaster genome
MOTIVATION In eukaryotes, the family of non-coding RNA genes includes a number of genes encoding small nucleolar RNAs (mainly C/D and H/ACA snoRNAs), which act as guides in the maturation or post-transcriptional modifications of target RNA molecules. Since in Drosophila melanogaster (Dm) only few examples of snoRNAs have been identified so far by cDNA libraries screening, integration of the mol...
متن کاملRNA Interference (RNAi) Screening in Drosophila
In the last decade, RNA interference (RNAi), a cellular mechanism that uses RNA-guided degradation of messenger RNA transcripts, has had an important impact on identifying and characterizing gene function. First discovered in Caenorhabditis elegans, RNAi can be used to silence the expression of genes through introduction of exogenous double-stranded RNA into cells. In Drosophila, RNAi has been ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009